Aberration measurement and correction on a large field of view in fluorescence microscopy

• Published in: arXiv.org
• Main Authors: Furieri, Tommaso, Ancora, Daniele, Calisesi, Gianmaria, Morara, Stefano, Bassi, Andrea, Bonora, Stefano
• Format: Paper
• Language: English
• Published: Ithaca Cornell University Library, arXiv.org 03.06.2021
• Subjects: Aberration; Algorithms; Closed loops; Deformable mirrors; Deformation; Field of view; Fluorescence; Formability; Lenses; Light sheets; Microscopes; Microscopy; Modules; Optical microscopes; Optimization; Plug & play; Sample holders; Wave front reconstruction; Wave fronts

The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the complexity of these systems, the level of correction is oft...