Aberration measurement and correction on a large field of view in fluorescence microscopy

The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the complexity of these systems, the level of correction is oft...

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Bibliographic Details
Published inarXiv.org
Main Authors Furieri, Tommaso, Ancora, Daniele, Calisesi, Gianmaria, Morara, Stefano, Bassi, Andrea, Bonora, Stefano
Format Paper
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 03.06.2021
Subjects
Aberration
Algorithms
Closed loops
Deformable mirrors
Deformation
Field of view
Fluorescence
Formability
Lenses
Light sheets
Microscopes
Microscopy
Modules
Optical microscopes
Optimization
Plug & play
Sample holders
Wave front reconstruction
Wave fronts
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Summary:The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the complexity of these systems, the level of correction is often limited to a small area in the field of view of the microscope. In this work, we present a plug and play module for aberration measurement and correction. The wavefront correction is performed through direct wavefront reconstruction using the spinning-pupil aberration measurement and controlling a deformable lens in closed loop. The lens corrects the aberrations in the center of the field of view, leaving residual aberrations at the margins, that are removed by anisoplanatic deconvolution. We present experimental results obtained in fluorescence microscopy, with a wide field and a light sheet fluorescence microscope. These results indicate that detection and correction over the full field of view can be achieved with a compact transmissive module placed in the detection path of the fluorescence microscope.
ISSN:2331-8422