Cortical actin properties controlled by Drosophila Fos aid macrophage infiltration against surrounding tissue resistance

• Published in: bioRxiv
• Main Authors: Belyaeva, Vera, Wachner, Stephanie, Gyoergy, Attila, Shamsi Emtenani, Gridchyn, Igor, Akhmanova, Maria, Linder, Markus, Roblek, Marko, Sibilia, Maria, Siekhaus, Daria
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 28.09.2021
• Subjects: Actin; Cytoskeleton; Drosophila; Hemocytes; Infiltration; Insects; Macrophages; Mechanical properties; Metastases; Plasmatocytes; Sequence analysis; Tumors
• DOI: 10.1101/2020.09.18.301481

The infiltration of immune cells into tissues underlies the establishment of tissue resident macrophages, and responses to infections and tumors. Yet the mechanisms immune cells utilize to negotiate tissue barriers in living organisms are not well understood, and a role for cortical actin has not been examined. Here we find that the tissue invasion of Drosophila macrophages, also known as plasmatocytes or hemocytes, utilizes enhanced cortical F-actin levels stimulated by the Drosophila member of the fos proto oncogene transcription factor family (Dfos, Kayak). RNA sequencing analysis and live imaging show that Dfos enhances F-actin levels around the entire macrophage surface by increasing mRNA levels of the membrane spanning molecular scaffold tetraspanin TM4SF, and the actin cross-linking filamin Cheerio which are themselves required for invasion. Cortical F-actin levels are critical as expressing a dominant active form of Diaphanous, a actin polymerizing Formin, can rescue the Dfos Dominant Negative macrophage invasion defect. In vivo imaging shows that Dfos is required to enhance the efficiency of the initial phases of macrophage tissue entry. Genetic evidence argues that this Dfos-induced program in macrophages counteracts the constraint produced by the tension of surrounding tissues and buffers the mechanical properties of the macrophage nucleus from affecting tissue entry. We thus identify tuning the cortical actin cytoskeleton through Dfos as a key process allowing efficient forward movement of an immune cell into surrounding tissues. Competing Interest Statement The authors have declared no competing interest. Footnotes * We showed that the two Fos targets, the filamin, Cheerio, and the tetraspanin TM4SF lead to increased Rho1 activation and Dia localization at the cortex. See new panels Fig 4M-P, S4E-F Fig. We also demonstrated that expressing DiaCA in macrophages could rescue the macrophage invasion defect in the null and hypmorphic Dfos alleles (S4B Fig). We also inserted single plane insets to show cortical actin more clearly in 4B-C, F-H. New control S4A,A'.