In vitro evaluation of collagen cross-linkers on dentin bond strength

• Main Author: Al-Ammar, Aiman A
• Format: Dissertation
• Language: English
• Published: ProQuest Dissertations & Theses 01.01.2008
• Subjects: Dentistry
• ISBN: 9781109023688; 1109023685

A dentin bonded restoration is made of the dentin, the hybrid layer, and the adhesive. Type I collagen is a major component of the hybrid layer, and improving its mechanical properties may be advantageous during bonding procedures. Since tensile forces contribute to clinical failures of bonded restorations, tensile bond testing is an alternative way of measuring the bond strength between the adhesive and dentin. Many studies have been conducted to improve and evaluate new adhesive systems. Collagen cross-linkers have been shown to improve the mechanical properties of dentin. However, the effect of a collagen cross-linking agent on the dentin bond strength has not been investigated. Therefore, this study was conducted to evaluate the effect of three different cross-linking agents (Glutaraldehyde [GD], Genipin [GE], and Grape Seed Extract [GSE]) on the dentin bond strength after three aging methods (24 hours at 37°C, proteolyctic challenge with 2.5% NaOCl treatment for 4 hours and 6 months distilled water storage at 37°C). Sixty-four sound human molars were collected and their occlusal surfaces were ground flat to expose dentin. Dentin surfaces were etched and randomly divided according to the dentin treatment: control group, 5% GD, 6.5% GSE and 0.5% GE. The teeth were then kept in their respective solutions for 1 hour and later restored with either One Step Plus (Bisco) or Adper Single Bond Plus (3M ESPE) adhesive systems. After 24 hours, the teeth were sectioned into hour glass shape beams to produce a cross-sectional surface area of 1.0 mm2.