Purine auxotrophic starvation evokes quiescence like phenotype in the budding yeast

Purine auxotrophy is a typical marker for many laboratory yeast strains. Supplementation of additional purine source (like adenine) is necessary to cultivate these strains. If not supplied in adequate amounts, purine starvation sets in. We tested purine starvation effects in budding yeast Saccharomy...

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Published inbioRxiv
Main Authors Kokina, Agnese, Ozolina, Zane, Pleiko, Karlis, Svirksts, Karlis, Tanilas, Kristel, Vamza, Ilze, Liepins, Janis
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 14.05.2021
Subjects
Adenine
AMP
Auxotrophy
Cell cycle
Culture media
Desiccation
Energy charge
Gene expression
Intermediates
Intracellular
Metabolic response
Metabolism
Phenotypes
Purines
rRNA
Stationary phase
Supplements
Transcription
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Yeast
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Summary:Purine auxotrophy is a typical marker for many laboratory yeast strains. Supplementation of additional purine source (like adenine) is necessary to cultivate these strains. If not supplied in adequate amounts, purine starvation sets in. We tested purine starvation effects in budding yeast Saccharomyces cerevisiae ade8 knockout. We explored effects brought by purine starvation in cellular, central carbon metabolism and in the global transcriptome level. We observed that cells cultivated in purine depleted media became significantly more tolerant to severe thermal, oxidative and desiccation stresses when compared to the cells cultivated in media with all necessary supplements. When starved for purine, cells stop their cell cycle in G1 or G0 state; intracellular concentration of ATP, ADP and AMP decreases, but adenylate charge remains stable. Intracellular RNA concentration decreases and massive downregulation of ribosomal RNA occurs. We think that purine auxotrophic starvation in a way mimics natural nitrogen or carbon starvations and therefore initiates elements of a transcriptional program typical for stationary phase cells (cell cycle arrest, increased stress resistance). Therefore our results demonstrate that organised metabolic response is initiated not only via natural starvations, but also when starving for metabolic intermediates, like purines. Competing Interest Statement The authors have declared no competing interest. Footnotes * Last passage in conclusions updated with latest literature
DOI:10.1101/2021.03.15.435510