Quantitative and qualitative analysis of interferon stimulated genesMX2, OAS1, and ISG15 for detection of pregnancy in cattle

Pregnancy in cattle can be determined using transrectal ultrasonography or by detection of pregnancy associated glycoproteins present in maternal circulation begging at approximately d 28 of gestation; however, the majority of pregnancy loss occurs prior to this date. Interferon stimulated genes (IS...

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Bibliographic Details
Published inJournal of animal science Vol. 98; p. 44
Main Authors DeCarlo, Andrea N, Parrish, Joseph, Long, Nathan, Pratt, Scott
Format Journal Article
LanguageEnglish
Published Champaign Oxford University Press 01.11.2020
Subjects
Blood
Cattle
Estrus
Genes
Gestation
Glycoproteins
Interferon
Pregnancy
Qualitative analysis
Qualitative research
Quantitative analysis
Ribonucleic acid
RNA
Synchronism
Synchronization
Ultrasound
Variance analysis
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Summary:Pregnancy in cattle can be determined using transrectal ultrasonography or by detection of pregnancy associated glycoproteins present in maternal circulation begging at approximately d 28 of gestation; however, the majority of pregnancy loss occurs prior to this date. Interferon stimulated genes (ISG) have been observed to be upregulated in pregnant cattle and measurable at d 18 of gestation. The objective of this study was to assess ISG expression as an indicator of pregnancy at d 18 post-insemination. A 7-d Co Synch and CIDR estrous synchronization protocol was utilized on cows of varying age and parity (n = 80) and insemination to a single sire performed 60 to 66 h following removal of the CIDR. On d 0 and d 18 post-insemination, blood was collected via jugular venipuncture for isolation of total RNA by PAXgene Blood RNA kit (PreAnalytiX, Hombrechtikon, Switzerland). Pregnancy detection was performed on d 30 of gestation by transrectal ultrasonography. Differences in ISG expression between d 0 and d 18 samples in both pregnant (n = 10) and open (n = 10) cows, according to d 30 ultrasound, were assessed. Primers for ISGs (MX2, OAS1, and ISG15) and housekeeping, cyclophilin were generated for use in end-point PCR and qRT-PCR. Data for qRT-PCR were normalized to cyclophilin expression. Analysis of variance and pair-wise student's t-test among LSMeans were performed with fixed effects for pregnancy status, day, and their interactions. End-point products in all ISGs, regardless of day or pregnancy status, were observed. Expression of MX2 and ISG15 were upregulated in open cows and in d 0 samples (P < 0.05). Expression of OAS1 was increased in open cows (P = 0.001). These data disagree with previous findings in which ISGs were upregulated in pregnant animals, and indicate expression of these ISGs may not be good indicators for pregnancy.
ISSN:0021-8812
1525-3163