The genetic relation of Indonesian Calloselasma rhodostoma based on ND4 gene and preliminary study of its venom storage condition

Calloselasma rhodostoma (Kuhl, 1824) is one species of Indonesian medically important snake that distributed in many Indonesia regions. With the recent snakebite cases of this snake and warrant of further improvement on anti-venin in Indonesia, the information on storage conditions for its venom is...

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Bibliographic Details
Published inEurasian journal of biosciences Vol. 14; no. 2; pp. 3359 - 3366
Main Authors Kadafi, Ahmad Muammar, Chrestella, Dea Jolie, Fatchiyah, Kurniawan, Nia
Format Journal Article
LanguageEnglish
Published Izmir Foundation for Environmental Protection and Research, Çevre Koruma ve Araştırma Vakfı (ÇEVKOR) 01.01.2020
Subjects
Bayesian analysis
Calloselasma rhodostoma
Deoxyribonucleic acid
DNA
Enzymes
Genetic relationship
ND4 gene
Phylogenetics
Phylogeny
Population
Proteins
Snake bites
Snakes
Species
Storage conditions
Venom
Borneo
Thailand
Indonesia
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Summary:Calloselasma rhodostoma (Kuhl, 1824) is one species of Indonesian medically important snake that distributed in many Indonesia regions. With the recent snakebite cases of this snake and warrant of further improvement on anti-venin in Indonesia, the information on storage conditions for its venom is important for developing an anti-venin. The genetic identity that possibly holds a cryptic diversity that has not been resolved, could impact the future of anti-venin development of this species. We analyze the molecular data based on the ND4 gene to resolve the genetic relations of this snake from Java, Kangean, and Borneo population with the addition of the Thailand population by Bayesian Inference phylogenetic reconstruction. To evaluate the storage conditions, venom collection from six living specimens from the Java population was used to analyze the effect of svPLA2 activity under different storage conditions for 14 days long. The phylogenetic results show a polytomy tree, with a low p-distance value between populations. Only the storage at 37 °C affects the performance of svPLA2 significantly. The phylogenetic indicating a single species even though divided by geographical barriers, more genes need to be compared to resolve the genetic relationship. More samples are needed to compare the venom properties throughout C. rhodostoma wide distribution, to enlight the anti-venin future development.
ISSN:1307-9867