Effects of Silver Nanoparticles on Primary Cell Cultures ofFibroblasts and Keratinocytes in a Wound-Healing Model

• Published in: Journal of applied biomaterials & functional materials Vol. 14; no. 2; pp. 137 - 142
• Main Authors: Franková Jana Dr, Pivodová Veronika, Vágnerová Hana, Juráňová Jana, Ulrichová Jitka
• Format: Journal Article
• Language: English
• Published: Thousand Oaks Sage Publications Ltd 01.04.2016
• Subjects: Angiogenesis; Chemokines; Colony-stimulating factor; Cyclooxygenase-2; Cytokines; Fibroblasts; Gelatinase A; Gelatinase B; Granulocyte-macrophage colony-stimulating factor; Growth factors; Inflammation; Interleukin 12; Interleukin 6; Interleukin 8; Interstitial collagenase; Keratinocytes; Matrix metalloproteinase; Nanoparticles; Parameters; Production methods; Silver; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vascular endothelial growth factor; Wound healing
• ISSN: 2280-8000
• DOI: 10.5301/jabfm.5000268

Background Nanoparticles are widely used in different technological fields, one of whichis medicine. Because of their antibacterial properties, silver nanoparticles(AgNPs) are used in several types of wound dressings for the treatment ofburns and nonhealing wounds, but their influence on each component of thewound-healing process remains unclear. In the present study, we evaluatedthe effects of AgNPs on normal human dermal fibroblasts (NHDFs) and normalhuman epidermal keratinocytes (NHEKs). Both cell types are important forwound healing, including with regard to inflammation, proliferation andtissue remodeling. Each phase of wound healing can be characterized by thesecretion of cytokines, chemokines and growth factors. Methods The production of inflammatory parameters (tumor necrosis factor α [TNF-α],interleukin-6 [IL-6], IL-8 and IL-12 and cyclooxygenase-2 [COX-2]),angiogenesis parameters (vascular endothelial growth factor [VEGF],granulocyte macrophage colony-stimulating factor) and matrixmetalloproteinases (MMP-1, MMP-2, MMP-3 and MMP-9) by NHDFs and NHEKs wereexamined by ELISA or Western blot after 24 and 48 hours of incubation withAgNPs. Results We found that AgNPs decreased some inflammatory cytokines (TNF-α and IL-12)and growth factors (VEGF) that were produced by NHDFs and NHEKs after 24 and48 hours and decreased the expression of COX-2 after 24 hours but only atthe highest concentration of AgNPs (25 parts per million). Conclusions The results indicate that NHEKs are more susceptible to the application ofAgNPs than NHDFs, and AgNPs may be useful for medical applications for thetreatment of wounds.