Assessment of micronuclei frequency in exfoliated buccal mucosal cells in Type-2 diabetes patients with tobacco chewing habit: A histopathologic study

Background: Detection of morphological and nuclear abnormalities of the cell are the basis of cytological evaluation and for that, micronucleus frequency evaluation in buccal exfoliative cells is a preferable method for cancer risk assessment because it is less technique sensitive, easy and an appre...

Full description

Saved in:
Bibliographic Details
Published inJournal of Advanced Medical and Dental Sciences Research Vol. 8; no. 3; pp. 78 - 81
Main Authors Husain, Fasahat, Sreedhar, Gadiputi, George, Jiji, Shukla, Abhilasha, Rehan, Ahmad Ahmad, Singh, Ankita
Format Journal Article
LanguageEnglish
Published Amritsar Journal of Advanced Medical and Dental Sciences Research (JAMDSR) 01.03.2020
Subjects
Abnormalities
Binding sites
Biomarkers
Cell division
Chewing
Chromosomes
Deoxyribonucleic acid
Diabetes
Diabetes mellitus
DNA
DNA damage
Evaluation
Free radicals
Frequency analysis
Genomic instability
Habits
Health risks
Hyperglycemia
Insulin
Mastication
Micronuclei
Morphology
Mucosa
Nitrogen
Oral cancer
Oxidation
Oxidative stress
Power
Risk assessment
Software
Stains & staining
Tobacco
Tobacco smoking
India
Online AccessGet full text

Cover

More Information
Summary:Background: Detection of morphological and nuclear abnormalities of the cell are the basis of cytological evaluation and for that, micronucleus frequency evaluation in buccal exfoliative cells is a preferable method for cancer risk assessment because it is less technique sensitive, easy and an appreciable biomarker for genomic instability. Hence; under the light of above mentioned data, the present study was undertaken for evaluating micronuclei frequency in exfoliated buccal mucosal cells in Type-2 diabetes patients with tobacco chewing habit. Materials & methods: A total of 75 subjects were enrolled and were divided into three study groups as follows: Group A: 25 Diabetic with tobacco chewing habit, Group B: 25 Non-diabetic with tobacco chewing habit, and Group C: 25 Controls. The cyto smears was separately stained with PAP and GIEMSA stains. The slides were mounted with cover glass using DPX mountant and were analysed. Average frequency of MN= Total no of MN/ Total no of cells with MN. Recording of all the results was done in Microsoftexcel sheet and were analysed by SPSS software. Chi- square test was used for evaluation of level of significance. Results: Mean MF frequency per high power field by GIEMSA stain among subjects of group A, Group B and Group C was found to be 1.19, 0.98 and 0.69 respectively. Significant results were obtained while comparing the mean MN frequency per high power field in GIEMSA stain and PAP stain. Conclusion: Type-2 diabetic patients with habits of tobacco smoking had significantly higher frequency of Micronuclei as compared to patients with diabetes and control group.
ISSN:2321-9599
DOI:10.21276/jamdsrz