Chemotaxis in polycyclic aromatic hydrocarbon-degrading bacteria isolated from coal-tar- and oil-polluted rhizospheres

The limited mass transfer in polycyclic aromatic hydrocarbon (PAH)-contaminated soils during bioremediation treatments often impedes the achievement of regulatory decontamination end-points. Little is known about bioavailability of these hydrophobic pollutants in phytoremediation systems. This work...

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Bibliographic Details
Published inFEMS microbiology ecology Vol. 44; no. 3; pp. 373 - 381
Main Authors Ortega-Calvo, J J, Marchenko, AI, Vorobyov, A V, Borovick, R V
Format Journal Article
LanguageEnglish
Published Delft Oxford University Press 01.06.2003
Subjects
Anthracene
Aromatic hydrocarbons
Bacteria
Bioavailability
Bioremediation
Chemotactic response
Chemotaxis
Coal tar
Decontamination
Degradation
Densitometers
Ecology
Exudates
Exudation
Hydrocarbon-degrading bacteria
Hydrocarbons
Hydrophobicity
Isotopes
Lipopolysaccharides
Mass transfer
Microbiology
Naphthalene
Oil pollution
Phenanthrene
Phytoremediation
Pollutants
Polycyclic aromatic hydrocarbons
Pyrene
Rhizosphere
Sediment pollution
Soil contamination
Soil pollution
Soil treatment
Soils
Strains (organisms)
Tar
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Summary:The limited mass transfer in polycyclic aromatic hydrocarbon (PAH)-contaminated soils during bioremediation treatments often impedes the achievement of regulatory decontamination end-points. Little is known about bioavailability of these hydrophobic pollutants in phytoremediation systems. This work attempts to evaluate, for the first time, chemotaxis as a bioavailability-promoting trait in PAH-degrading bacteria from the rhizosphere. For this aim, 20 motile strains capable of degrading different PAHs were isolated from rhizosphere soils contaminated with coal tar and oil. Three representative Pseudomonas strains were selected, on the basis of their faster growth and/or range of PAHs degraded, for detailed chemotaxis studies with PAHs (naphthalene, phenanthrene, anthracene, and pyrene), bacterial lipopolysaccharide and root exudates from seven different plants. The chemotactic response was quantified with a new densitometric method. The results indicate that chemotaxis is a relevant mobilizing factor for PAH-degrading rhizosphere bacteria.
ISSN:0168-6496
1574-6941
DOI:10.1016/S0168-64960300092-8