PATHOLOGY Analysis of the Canine mdr1-1[Delta] Mutation in the Dog Breed Elo

• Published in: Transboundary and emerging diseases Vol. 54; no. 8; p. 401
• Main Authors: Fecht, S, Wöhlke, A, Hamann, H, Distl, O
• Format: Journal Article
• Language: English
• Published: Oxford Hindawi Limited 01.10.2007
• Subjects: Dogs; Drug resistance; Mutation; Veterinary services
• ISSN: 1865-1674; 1865-1682
• DOI: 10.1111/j.1439-0442.2007.00966.x

With 1 figure and 1 table A deletion mutation in the canine multidrug resistance gene, MDR1, is associated with drug sensitivity. This was shown for several purebred dog breeds from the Collie lineage such as the Collie (rough-coated and smooth-coated), the Australian Shepherd and the Old English sheepdog. To determine whether the mdr1-1[Delta] mutation could be found in the newly bred German dog breed Elo which is based amongst other breeds on Old English sheepdogs, 177 blood samples representative for the Elo breed were collected. After DNA extraction, a polymerase chain reaction-based method with subsequent polyacrylamide gel electrophoresis was used for detection of the mdr1-1[Delta] mutation. The mdr1-1[Delta] allele was not observed in the Elos investigated. The probability that the mdr1-1[Delta] allele originated in the Old English sheepdog breed is segregating in the Elo population was estimated at 3.68 x 10-17. [PUBLICATION ABSTRACT]