Affinity sorption analysis of the structure of poly(G)·poly(C) complex obtained by means of template-dependent synthesis

The analysis of the structure of poly(G)·poly (C) complexes obtained by the enzymatic synthesis on poly(C) template was carried out using the method of affinity sorption. This latter was used also for the purification of the synthesized so far complexes from the redundant template as well as for the...

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Bibliographic Details
Published inBiopolimery i kletka Vol. 9; no. 3; p. 23
Main Authors Surzhik, M A, Timkovsky, A L
Format Journal Article
LanguageEnglish
Published Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 01.01.1993
Subjects
Affinity
DNA-directed RNA polymerase
Enzymatic synthesis
Purification
RNA polymerase
Sorption
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Summary:The analysis of the structure of poly(G)·poly (C) complexes obtained by the enzymatic synthesis on poly(C) template was carried out using the method of affinity sorption. This latter was used also for the purification of the synthesized so far complexes from the redundant template as well as for the investigation of the structure of poly(G) ·poly(C) complexes obtained by the «traditional» mixing of complementary homopo-lynucleotides. It was shown that the degree of template transcription depends on the quality of RNA polymerase and the incompleted transcription is connected with the inhibition of the elongation of poly(G) chain.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000356