Multitrate Phosphate Solubilizing Bacteria from Dalbergia sissoo Roxb. Rhizosphere in Natural Forests of Indian Central Himalayas

Plant growth promoting rhizobacteria (PGPR) are the rhizosphere bacteria that may be directly or indirectly utilized to augment plant growth. Dalbergia sissoo Roxb. (common name shisham) is a leguminous tree known internationally for its highly valued timber. The objectives of this study were to cha...

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Bibliographic Details
Published inEnvironment and ecology Vol. 37; no. 3A; p. 894
Main Authors Joshi, Samiksha, Jaggi, Vandana, Tiwari, Salil, Sah, V K, Sahgal, Manvika
Format Journal Article
LanguageEnglish
Published Kalyani MKK Publications 01.07.2019
Subjects
Ammonia
Bacteria
Dalbergia sissoo
Enzymatic activity
Enzyme activity
Enzymes
Forests
Host plants
Klebsiella
Lipase
Micronutrients
Morphology
Nutrient availability
Nutrients
Organic carbon
Pectinase
Phosphates
Phosphorus
Plant growth
Plants (botany)
Rhizosphere
rRNA 16S
Soil nutrients
Soils
Solubilization
Timber
Urease
Vernacular names
Xylanase
Zinc
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Summary:Plant growth promoting rhizobacteria (PGPR) are the rhizosphere bacteria that may be directly or indirectly utilized to augment plant growth. Dalbergia sissoo Roxb. (common name shisham) is a leguminous tree known internationally for its highly valued timber. The objectives of this study were to characterize bacterial diversity indigenous to D. sissoo rhizosphere in natural forests located at Lachhiwala, Tanakpur and Pantnagar in Uttarakhand. Rhizospheric soil from all three sites was analyzed for biotic (viz. total viable bacterial count (TVC) and soil enzyme activities) as well as abiotic (pH, organic carbon, EC and available N, P, K and micronutrients) properties. Highest bacterial count was recorded at Tanakpur followed by Lachhiwala and Pantnagar. A strong positive correlation was observed between all the enzyme activities and soil nutrients (macro and micro). Since phosphatase enzyme activity and available phosphate status in soil at all sites was higher. Therefore, phosphate solubilizing bacteria (PSB) were recovered and characterized. In addition, all isolates exhibited one or more enzymatic (amylase, lipase, pectinase, protease, urease, xylanase) and plant growth promoting (PGP) traits like the production of siderophore, HCN, IAA production, ammonia and solubilization of zinc and phosphorus. Out of all, strain L8 from Lacchiwala and P2 from Pantnagar showed a maximum number of PGP-positive traits. Amongst 18 isolates, seven were identified as Pseudomonas sp., four as Streptomyces sp., two as Klebsiella sp., two as Staphylococcus sp. and one each as Pantoea sp., Kitasatospora sp. and Micrococcus sp. based on morphology and 16S rDNA sequence analysis. These results enhance our knowledge of bacterial diversity in shisham rhizosphere. Moreover, Since these isolates exhibited multiple traits beneficial to the host plants, they can serve as potential bioinoculant candidates for the soil-plant system to increase their growth as well as productivity.
ISSN:0970-0420