Apport de l'algorithme d'EUCAST dans le criblage des carbapénèmase: Expérience du laboratoire de microbiologie au CHU Ibn Rochd Casablanca

Introduction: Carbapenemase is a bactericidal antibiotic. They are first line treatments for severe infections, especially those caused by Gram negative Multidrug Resistant Bacteria (BMRs), such as spectrum extended spectrum beta lactamase Enterbacteriaceae, several phenotypic techniques have been p...

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Bibliographic Details
Published inInternational journal of innovation and applied studies Vol. 25; no. 4; pp. 1290 - 1296
Main Authors Sakalli, H El Bayed, Touhami, K Ouazzani, Katfy, K, Zerouali, K, Belabbes, H
Format Journal Article
LanguageFrench
Published Rabat International Journal of Innovation and Applied Studies 01.03.2019
Subjects
Algorithms
Antibiotics
Enterobacter cloacae
Epidemiology
Infections
Klebsiella
Laboratories
Microbiology
Screening
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Summary:Introduction: Carbapenemase is a bactericidal antibiotic. They are first line treatments for severe infections, especially those caused by Gram negative Multidrug Resistant Bacteria (BMRs), such as spectrum extended spectrum beta lactamase Enterbacteriaceae, several phenotypic techniques have been proposed for the rapid detection of OPCs. In this work we will establish a comparaison of the performances of the two phenotypic methods of detection of carbapenemases: the Modified Hodge test and EUCAST algorithm. Results: A total of 18 enterobacterial strains were identified. The iodentification by the Api 10S gallery showed: a predominance of Klebsiella pneumoniae with a rate of 48%, followed by Enterbacter cloacae with a rate of 30%. The detection of carbapenemase production in the 18 enterobacterial strains was first performed by the Modified Hodge test, It revealed 83%. positivity and 17% negativity, the screening algorithm applied to 18 islates of the collected EPCs, showed a pourcentage of positivity of 78% which is more significant than the percentage of negativity 22%. The PCR reaction in final time allowed us to detect 15/18 strains of enterobacteriaceae producing genes encoding carbapenemases and therefore a percentage of positivity of 83,34%. This percentage of positivity with the use of real-time PCR 88,89%, detecting an increased strain of EPC more. Conclusion: At the end of our work, the screening algorithm proposed by EUCAST is the detection of EPCs dating from a good sensitivity and specificity according to the recommandations of learned societies.
ISSN:2028-9324