Impact of ¿^sub 1^-adrenoceptor expression on contractile properties of vascular smooth muscle cells

Low-frequency blood pressure oscillations (Mayer waves) are discussed as a marker for sympathetic modulation of vascular tone. However, the factors that determine the frequency response of the vasculature to sympathetic stimuli are not fully understood. Possible mechanisms include functions related...

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Published inAmerican journal of physiology. Regulatory, integrative and comparative physiology Vol. 293; no. 3; p. R1215
Main Authors Gericke, Adrian, Martinka, Peter, Nazarenko, Irina, Persson, Pontus B, Patzak, Andreas
Format Journal Article
LanguageEnglish
Published Bethesda American Physiological Society 01.09.2007
Subjects
Blood pressure
Cells
Hormones
Muscular system
Nervous system
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Summary:Low-frequency blood pressure oscillations (Mayer waves) are discussed as a marker for sympathetic modulation of vascular tone. However, the factors that determine the frequency response of the vasculature to sympathetic stimuli are not fully understood. Possible mechanisms include functions related to α...-adrenergic receptors (α...-AR) and postreceptor processes involved in the vascular contractile response. The purpose of the present study was to examine the hypothesis that expression levels of α...-AR and their subtype distribution determine velocity and magnitude of α...-AR-mediated vascular smooth muscle cell (VSMC) contraction. α..., α..., and α...-AR subtypes were transfected into VSMCs from rat aorta and characterized immunocytochemically via confocal microscopy. Functional studies in isolated cells were performed using video microscopy. The α...-AR agonist phenylephrine produced dose-dependent contractions of VSMCs. All transfected groups were more sensitive to phenylephrine compared with controls. Maximal contraction velocity almost doubled in transfected cells. However, no differences in observed parameters were found between the three transfected groups. Contractile properties in response to membrane depolarization with KCl were similar in all groups. In conclusion, α...-AR density determines velocity and sensitivity of α...-AR-mediated contraction in VSMCs. α...-AR subtype distribution does not appear to influence vasoconstriction to sympathetic stimuli. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0363-6119
1522-1490