Metaproteomics in large‐scale compost

• Published in: Microbial biotechnology Vol. 8; no. 6; pp. 950 - 960
• Main Authors: Liu, Dongming, Li, Mingxiao, Beidou Xi, Zhao, Yue, Zimin Wei, Song, Caihong, Zhu, Chaowei
• Format: Journal Article
• Language: English
• Published: Bedford John Wiley & Sons, Inc 01.11.2015
• Subjects: Bacteria; Biodegradation; Carbon; Cellulase; Cellulose; Composting; Composts; Deoxyribonucleic acid; DNA; Fungi; Gene expression; Metabolism; Microorganisms; Mineralization; Municipal solid waste; Municipal waste management; Nitrogen; Peptides; Phenols; Polymorphism; Proteins; Ribosomal DNA; Solid waste management; Solid wastes; Temperature; Thermophilic bacteria; Thermophilic fungi; Waste management; United States > US; China
• ISSN: 1751-7915
• DOI: 10.1111/1751-7915.12290

Composting is an appropriate management alternative for municipal solid waste; however, our knowledge about the microbial regulation of this process is still scare. We employed metaproteomics to elucidate the main biodegradation pathways in municipal solid waste composting system across the main phases in a large‐scale composting plant. The investigation of microbial succession revealed that Bacillales, Actinobacteria and Saccharomyces increased significantly with respect to abundance in composting process. The key microbiologic population for cellulose degradation in different composting stages was different. Fungi were found to be the main producers of cellulase in earlier phase. However, the cellulolytic fungal communities were gradually replaced by a purely bacterial one in active phase, which did not support the concept that the thermophilic fungi are active through the thermophilic phase. The effective decomposition of cellulose required the synergy between bacteria and fungi in the curing phase.