Helibobacter pylori detection in Chinese subjects: A comparison of two common DNA fingerprinting methods

This study aims to demonstrate DNA diversity in Helicobacter pylori strains obtained from patients with peptic ulcer disease and compare the results from two DNA fingerprinting techniques. H. pylori strains (n = 42), collected from June 1996 to August 1999, were cultured from storage at -80 degrees...

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Bibliographic Details
Published inBritish journal of biomedical science Vol. 58; no. 4; p. 239
Main Authors Yakoob, Javed, Hu, Guoling, Fan, Xuegong, Zhang, Zheng
Format Journal Article
LanguageEnglish
Published London Taylor & Francis Ltd 01.10.2001
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Summary:This study aims to demonstrate DNA diversity in Helicobacter pylori strains obtained from patients with peptic ulcer disease and compare the results from two DNA fingerprinting techniques. H. pylori strains (n = 42), collected from June 1996 to August 1999, were cultured from storage at -80 degrees C. DNA diversity was determined by the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis, and random amplified polymorphic DNA (RAPD)-PCR. Isolates showed a significant DNA sequence diversity by both methods. RAPD-PCR demonstrated 33 distinct strain-specific band patterns, whereas PCR-RFLP demonstrated six distinct restriction patterns. PCR-RFLP identified four of the 42 strains as mixed growth, compared with RAPD-PCR which did not reveal any co-colonisation. Diversity among H. pylori clinical isolates was demonstrated distinctly by both methods; however, RAPD-PCR showed greater discriminatory power in distinguishing between isolates.
ISSN:0967-4845
2474-0896