Association analysis of SNPs and diplotype of HSC70-1 gene with growth traits in largemouth bass (Micropterus salmoides)

In order to explore the effects of HSC70-1 gene on growth traits of largemouth bass(Micropterus salmoides) and develop growth-related SNPs, we obtained the cDNA of coding region and the complete sequence of HSC70-1 gene by PCR amplification.The encoding region cDNA sequence of HSC70-1 gene was 1 953...

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Bibliographic Details
Published inNanfang shuichan kexue no. 6
Main Authors Li, Shengjie, Fan, Jiajia, Jiang, Peng, Bai, Junjie, Sun, Jianguo, Wu, Jiankai, Fei, Zhiping
Format Journal Article
LanguageChinese
Published Guangzhou Chinese Academy of Fishery Sciences 01.01.2018
Subjects
Amino acids
Body height
Body mass
Complementary DNA
DNA
Freshwater fishes
Genotype & phenotype
Genotypes
Growth
Length
Micropterus salmoides
Nucleotide sequence
PCR
Sequencing
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Summary:In order to explore the effects of HSC70-1 gene on growth traits of largemouth bass(Micropterus salmoides) and develop growth-related SNPs, we obtained the cDNA of coding region and the complete sequence of HSC70-1 gene by PCR amplification.The encoding region cDNA sequence of HSC70-1 gene was 1 953 bp, encoding 650 amino acids. Its complete sequence was 3 390 bp in length, consisting of 8 exons and 7 introns. We screened four SNPs(at 821, 1 105, 1 200 and 2 804 bp on the introns) by direct sequencing, and found that the individuals with CC genotype of C-821G site had significantly higher values of body mass or total length than those with CG or GG genotypes(P0.05). At the position of A-2804 T, the individuals with TT genotype had significantly higher values of body mass or total length than those with AT or AA genotypes(P0.05). The other two SNP loci were not significantly correlated with growth traits(P0.05). The optical combined genotype of HSC70-1 gene was D1(CC--CCTT), which had significantly higher valu
ISSN:2095-0780