Cytotoxic Activity of Alkaloids Extracted from Trigonella foenum graecum (Fenugreek) against Breast Cancer Cell Line

• Published in: Journal of pharmaceutical sciences and research Vol. 11; no. 4; pp. 1662 - 1666
• Main Authors: AL-Sallami, Alaauldeen S M, Al-Labban, Zuhair Salih, Ali, Sanaa Mohemmed
• Format: Journal Article
• Language: English
• Published: Cuddalore Journal of Pharmaceutical Sciences and Research 01.04.2019
• Subjects: Apoptosis; Breast cancer; Chromatography; Cytotoxicity; Fibroblasts; Herbal medicine; Scientific imaging; Seeds; Iraq
• ISSN: 0975-1459

The proliferation rate was measured as the formula according to [17] as follows: PR% =B/A x 100 PR=proliferation rate, A= the optical density of control, B= the optical density of test Statistical analysis Data were expressed as means ± S.E. Statistical analyses were performed using graghpad prism program followed by LSD test. The results showed no significant (P > 0.05 ) toxic effect in the three cancer cells lines and the percentage of cytotoxicity effect of cell proliferations especially in high concentration used in present study (50000 -100000 pg/ml) Discussion Medicinal plants contain some organic compounds which provide definite physiological action on the human body, and these bioactive substances include alkaloids [18]. Total alkaloids were detected by changing the color of specialized reagents .The method described in this study can be used for the determination of a Cytotoxicity of total alkaloid against cell lines performed using a crystal violet assay, this methods describes a quick and reliable screening method that is suitable for the examination of the impact of chemotherapeutics or other compounds on cell survival and growth inhibition [19]. GC-MASS analysis also showed two anticancer compound in crude alkaloid such as Quinoline that plays an important role in anticancer drug development as their derivatives have shown excellent results through different mechanism of action such as growth inhibitors by cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness[31] Also compound is Indole are natural products which are well known for its anti-cancer activity due to its ability to induce cell death for many cancer cell lines[30] Indoles function as efficient group of numerous biochemical molecules and compounds, such as alkaloids.