Authors’ Response

Microscopists can easily measure incident light power with a 10× lens as was done in the paper and then divide that number by the exposure time to calculate the “temporal light dose.” [...]a longer exposure time would not result in reduced photo-toxicity and would not improve cellular health. [...]i...

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Bibliographic Details
Published inMicroscopy today Vol. 26; no. 2; pp. 58 - 59
Main Authors Mubaid, Firas, Brown, Claire M
Format Magazine Article
LanguageEnglish
Published Oxford Oxford University Press 01.03.2018
Subjects
Cell adhesion & migration
Exposure
Incident light
Light
Noise generation
Toxicity
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Summary:Microscopists can easily measure incident light power with a 10× lens as was done in the paper and then divide that number by the exposure time to calculate the “temporal light dose.” [...]a longer exposure time would not result in reduced photo-toxicity and would not improve cellular health. [...]instead of the “temporal light dose” parameter we would like to recommend that researchers keep the light power at a minimum and increase exposure time as much as required to generate a good signal-to-noise image. [...]if the data were collected so that the camera exposure time was kept constant and only the incident light power was changed, the exponential trend of the photo-bleaching rate with increasing power would be even more prominent.
ISSN:1551-9295
2150-3583
DOI:10.1017/S1551929518000275