Activation of hepaticstellate cells cocultured with hepatoma carcinoma cells in vitro and in vivo

• Published in: Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban no. 2; p. 227
• Main Authors: XIE Qun, Li-bin, LIN, Jin-tian, ZHANG, Xue-ming, YANG, Yang-yuan, LIN
• Format: Journal Article
• Language: Chinese
• Published: Xi'an Xi'an Jiaotong University 01.01.2019
• Subjects: Apoptosis; Liver cancer; Methods; Wound healing
• ISSN: 1671-8259
• DOI: 10.7652/jdyxb201902012

Objective: To investigate the activation of hepatic stellate cells (HSCs) cocultured with hepatoma carcinoma cells. Methods: MHCC97H cells and HSCs were cocultured by cell-cell contact method, fibrinogen-thrombin paste technique, conditioned media from MHCC97H(MHCC97H-CM) and Transwell coculture technique, respectively. MHCC97H cells and HSCs were inoculated s. c. into nude mice. The expression of α-SMA in HSCs was assessed by immunocytochemical staining and Western blotting. Proliferation and migration of HSCs was determined using Cell-Counting Kit-8 (CCK-8) and wound healing and Transwell technique, respectively. Results: The activation of HSCs was significantly increased in the all cocultured system. The expression of α-SMA was up-regulated by cell-cell contact method, MHCC97H-CM, Transwell coculture technique in vitro and in cancer-bearing mice in vivo. The increased chemotaxis of MHCC97H cells and HSCs was observed by cell-cell contact method, fibrinogen-thrombin paste technique and in cancer-bearing mic