Activation of hepaticstellate cells cocultured with hepatoma carcinoma cells in vitro and in vivo
Objective: To investigate the activation of hepatic stellate cells (HSCs) cocultured with hepatoma carcinoma cells. Methods: MHCC97H cells and HSCs were cocultured by cell-cell contact method, fibrinogen-thrombin paste technique, conditioned media from MHCC97H(MHCC97H-CM) and Transwell coculture tec...
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Published in | Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban no. 2; p. 227 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Chinese |
Published |
Xi'an
Xi'an Jiaotong University
01.01.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Objective: To investigate the activation of hepatic stellate cells (HSCs) cocultured with hepatoma carcinoma cells. Methods: MHCC97H cells and HSCs were cocultured by cell-cell contact method, fibrinogen-thrombin paste technique, conditioned media from MHCC97H(MHCC97H-CM) and Transwell coculture technique, respectively. MHCC97H cells and HSCs were inoculated s. c. into nude mice. The expression of α-SMA in HSCs was assessed by immunocytochemical staining and Western blotting. Proliferation and migration of HSCs was determined using Cell-Counting Kit-8 (CCK-8) and wound healing and Transwell technique, respectively. Results: The activation of HSCs was significantly increased in the all cocultured system. The expression of α-SMA was up-regulated by cell-cell contact method, MHCC97H-CM, Transwell coculture technique in vitro and in cancer-bearing mice in vivo. The increased chemotaxis of MHCC97H cells and HSCs was observed by cell-cell contact method, fibrinogen-thrombin paste technique and in cancer-bearing mic |
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ISSN: | 1671-8259 |
DOI: | 10.7652/jdyxb201902012 |