Quantification of the b2-Adrenergic Feed Additives Ractopamine and Salbutamol by Reductive Amination–Assisted Modification

• Published in: Journal of food protection Vol. 82; no. 4; pp. 696 - 702
• Main Authors: Shen, Po-Tsun, Wu, You-Ying, Chang, Yu-Tzu, Cheng, Chieh-Wen, Huang, Mei-Fang, Chen, Ziyu, Shiue, Yow-Ling, Liang, And Shih-Shin
• Format: Journal Article
• Language: English
• Published: Des Moines Elsevier Limited 01.04.2019
• Subjects: Amination; Analogs; Asthma; Biological materials; Calibration; Cattle; Chemists; Chromatography; Chronic obstructive pulmonary disease; Correlation coefficient; Correlation coefficients; Feed additives; Feeds; Food; Food additives; Food safety; Formaldehyde; Hogs; Ions; Livestock; Lung diseases; Mass spectrometry; Mass spectroscopy; Medical treatment; Muscle contraction; Muscles; Nanoparticles; Obstructive lung disease; Organic chemistry; Pork; Proteomics; Salbutamol; Scientific imaging; Smooth muscle; Sodium; Software; Urine; United States > US
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028XJFP-18-499

β2-Adrenergic compounds such as ractopamine and salbutamol can cause smooth muscle relaxation and are used for asthma and chronic obstructive pulmonary disease treatment. However, β2-adrenergic compounds may also cause some adverse cardiovascular effects or behavioral changes in humans; therefore- it is important for analytical chemists to determine the amounts of β2-adrenergic compounds in raw biological materials. Herein, novel analogs for standards and internal standard analogs were synthesized through reductive amination with isotopic H2-formaldehyde, D2-formaldehyde, and sodium cyanoborohydride. These analogs were used for quantifying β2-adrenergic compounds such as ractopamine and salbutamol in ground pork samples under multiple reaction monitoring scanning modes by tandem mass spectrometry. H2-Formaldehydemodified ractopamine and H2-formaldehyde-modified salbutamol acting as standards were used to prepare calibration curves, whereas D2-formaldehyde was used to generate D2-modified ractopamine and D2-modified salbutamol analogs acting as internal standards. The H2-modified ractopamine and H2-modified salbutamol showed excellent correlation coefficients, limits of detection, and limits of quantification. In mass spectrometric detection, H2-modified ractopamine showed 202% signal enhancement after modification and H2-modified salbutamol showed 17% signal enhancement after modification.