Effect of statins on ABCA1 gene expression in neurons and astrocytes / 他汀类药物对神经细胞和神经胶质细胞ABCA1基因表达的影响

Objective: To observe the effects of statins on ATP-binding cassette transporter 1 (ABCA1) gene expression in neurons and astrocytes. Methods: Primary human astrocytes and neuron cell line HCN-2 were incubated with simvastatin and pravastatin at different concentrations and under different condition...

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Published inXi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban no. 5; p. 527
Main Authors Hui-lin, GONG, Kuo, LI, Guan-jun, ZHANG, Hui-lian, HOU, Wei-jiang, DONG
Format Journal Article
LanguageChinese
Published Xi'an Xi'an Jiaotong University 01.01.2010
Subjects
ABC transporters
Gene expression
Neurons
Proteins
Statins
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Summary:Objective: To observe the effects of statins on ATP-binding cassette transporter 1 (ABCA1) gene expression in neurons and astrocytes. Methods: Primary human astrocytes and neuron cell line HCN-2 were incubated with simvastatin and pravastatin at different concentrations and under different conditions. Using RNA isolated from the cultured cells, we performed quantitative PCR to measure the ABCA1 gene expression in astrocytes and neurons. The protein expression of ABCA1 was measured by Western blot. Results: The two statins significantly decreased the ABCA1 gene and protein expressions. Compared with pravastatin, simvastatin significantly reduced the expression of ABCA1 in neurons and astrocytes by 95% and 75%, respectively (both P<0.05). The neurons were more sensitive than astrocytes. Conclusion: The statins are capable of decreasing the ABCA1 gene and protein expressions in neurons and astrocytes, and decreased ABCA1 expression may affect cholesterol metabolism in cells. The results may provide an important
ISSN:1671-8259