Significance of the quantitative analysis of Lactobacillus and Peptostreptococcus productus of gut microbiota in patients with type 2 diabetes / 2型糖尿病患者肠道乳酸菌属和消化链球菌属定量研究及其意义

• Published in: Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban no. 1; p. 93
• Main Authors: Xiao-kang, WU, Chao-feng, MA, Peng-bo, YU, Xiang-ling, WANG, HAN, Lei, Miao-xian, LI, Ji-ru, XU
• Format: Journal Article
• Language: Chinese
• Published: Xi'an Xi'an Jiaotong University 01.01.2015
• Subjects: Diabetes; Microbiota; Quantitative analysis
• ISSN: 1671-8259
• DOI: 10.7652/jdyxb201501017

Objective: To analyze quantitatively the intestinal Lactobacillus genus and Peptostreptococcus productus of patients with type 2 diabetes and healthy people to compare the differences of intestinal flora between the two groups of people. Methods: The total DNA of intestinal microbiota was extracted. The specific primers of bacterial genus were used for real-time fluorescent quantitative PCR amplification. And bacterial standard and standard curve were made. Then we analyzed quantitatively the content of two predominant bacterial genus in patients with type 2 diabetes and healthy people. Results: Compared with that in the healthy controls, the number of the intestinal Peptostreptococcus productus in the patients with type 2 diabetes was significantly reduced. However, as for the number of intestinal Lactobacillus genus, no significant differences were found between the two groups. Conclusion: Real-time PCR can be taken as an accurate and sensitive testing method to study the intestinal microbiota. The changes