A Screen for Schizosaccharomyces pombe Mutants Defective in Rereplication Identifies New Alleles of rad4^sup +^, cut9^sup +^ and psf2^sup

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cd...

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Bibliographic Details
Published inGenetics (Austin) Vol. 169; no. 1; p. 77
Main Authors Gómez, Eliana B, Angeles, Vanessa T, sburg, Susan L
Format Journal Article
LanguageEnglish
Published Bethesda Genetics Society of America 01.01.2005
Subjects
Deoxyribonucleic acid
DNA
Gene expression
Genes
Mutation
Proteins
Yeast
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Summary:Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9^sup +^, the initiation/checkpoint factor rad4^sup +^/cut5^sup +^, and the first mutant allele of psf2^sup +^, a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization. [PUBLICATION ABSTRACT]
ISSN:0016-6731
1943-2631