Serum-free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivityt

Stable E1 transformed cells, like PER.C6, are able to grow at scale and to high cell densities. E1-deleted adenoviruses replicate to high titer in PER.C6 cells whereas subsequent deletion of E2A from the vector results in absence of replication in PER.C6 cells and drastically lowers the expression o...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology and bioengineering Vol. 100; no. 2; p. 273
Main Authors Havenga, MJE, Holterman, L, Melis, I, Smits, S, Kaspers, J, Heemskerk, E, Vlugt, R van der, Koldijk, M, Schouten, GJ, Hateboer, G, Brouwer, K, Vogels, R, Goudsmit, J
Format Journal Article
LanguageEnglish
Published New York Wiley Subscription Services, Inc 01.06.2008
Subjects
Biotechnology
Cells
Genes
Proteins
Respiratory distress syndrome
Viruses
Online AccessGet full text

Cover

More Information
Summary:Stable E1 transformed cells, like PER.C6, are able to grow at scale and to high cell densities. E1-deleted adenoviruses replicate to high titer in PER.C6 cells whereas subsequent deletion of E2A from the vector results in absence of replication in PER.C6 cells and drastically lowers the expression of adenovirus proteins in such cells. We therefore considered the use of an ...E1/...E2 type 5 vector (Ad5) to deliver genes to PER.C6 cells growing in suspension with the aim to achieve high protein yield. To evaluate the utility of this system we constructed ...E1/...E2 vector carrying different classes of protein, that is, the gene coding for spike protein derived from the Goronavirus causing severe acute respiratory syndrome (SARS-CoV), a gene coding for the SARS-CoV receptor or the genes coding for an antibody shown to bind and neutralize SARS-CoV (SARS-AB). The ...E1/...E2A-vector backbones were rescued on a PER.C6 cell line engineered to constitutively over express the Ad5 E2A protein. Exposure of PER.C6 cells to low amounts (30 vp/cell) of ...E1/...E2 vectors resulted in highly efficient (>80%) transduction of PER.C6 cells growing in suspension. The efficient cell transduction resulted in high protein yield (up to 60 picogram/cell/day) in a 4 day batch production protocol. FACS and ELISA assays demonstrated the biological activity of the transiently produced proteins. We therefore conclude that ...E1/...E2 vectors in combination with the PER.C6 technology may provide a viable answer to the increasing demand for high quality, high yield recombinant protein. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0006-3592
1097-0290