Regiospecific Ester Hydrolysis by Orange Peel Esterase

Enzyme catalysis is an important topic in undergraduate bioorganic chemistry courses, but it presents problems to demonstrate effectively in a chemistry laboratory. As an accompaniment to a second year undergraduate course in bioorganic chemistry, Bugg et al develop a simple but effective experiment...

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Bibliographic Details
Published inJournal of chemical education Vol. 74; no. 1; p. 105
Main Authors Bugg, Timothy D H, Lewin, Andrew M, Catlin, Eric R
Format Journal Article
LanguageEnglish
Published Easton American Chemical Society 01.01.1997
Subjects
Catalysis
Chemical reactions
Chemistry
Citrus fruits
Enzymes
Experiments
Organic chemistry
Research Methodology
Science education
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Summary:Enzyme catalysis is an important topic in undergraduate bioorganic chemistry courses, but it presents problems to demonstrate effectively in a chemistry laboratory. As an accompaniment to a second year undergraduate course in bioorganic chemistry, Bugg et al develop a simple but effective experiment that demonstrates the regiospecificity of enzyme catalysis using an esterase activity easily isolated from orange peel and compare its specificity with that of two commercially available esterase enzymes. This experiment offers students the opportunity to see enzyme catalysis in action and to prepare an enzyme extract from a natural source.
ISSN:0021-9584
1938-1328