Evaluation of antibacterial activity of Fe^sub 2^O^sub 3^ nanoparticles against Shigella dysenteriae

• Published in: Journal of pharmaceutical sciences and research Vol. 10; no. 8; pp. 1980 - 1982
• Main Authors: Kareem, Zainab H, Shareef, Hasanain Khaleel, Alkaim, Ayad F
• Format: Journal Article
• Language: English
• Published: Cuddalore Journal of Pharmaceutical Sciences and Research 01.08.2018
• Subjects: Antibiotics; Antimicrobial agents; Bacteria; Bacterial infections; Cancer; Composite materials; Metal oxides; Nanocomposites; Nanomaterials; Nanoparticles; Pharmaceuticals; Researchers
• ISSN: 0975-1459

Abstract Fe2O3 nanoparticles were synthesized using Hydrothermal method, Ag+ ions on the Fe2O3 nanoparticles reduced to the metal form using a photodeposition method. [...]Ag/Fe3O4 composite core-shell nanoparticles were synthesized. Synthesis of Fe2O3/Ag Composite Two gram of Sodium Hydroxide (NaOH) was dissolved in 200 ml distilled water with constant stirring, then 1gm of FeCl3.6H2O was added to the solution and (...)by N2(Nitrogen gas) for 30 min with continuous stirring for 1-2 hrs to get a homogeneous solution followed by hydrothermal treatment in Teflon- lined stainless steel vessels for 24hrs in an oven that was preheated to 170°C. After the hydrothermal reaction, the products were cooled down to room temperature and the solid products inside the Teflon vessels were separated from the solution and washed several times with distilled water with ultrasonic device to remove any soluble salts until the pH of the wash became neutral(pH =7), the solid products were finally dried at 70 °C for 24 h in a dustproof environment prior to photodeposition of Ag on it. Ag/Fe2O3 nanocomposite was prepared by photo deposition of Ag on the surface of Fe2O3, 2gm from Fe2O3 nanoparticle and a proper amount (0.5%) of silver nitrate (AgNO3) were contained in a quartz cell, the distilled water and methanol with ratio 10 % v/v (H2O/ methanol) were added and pargulated by N2(Nitrogen gas) for 30 minute, then the system was irradiated with UVA light (wavelength 365nm and light intensity of 2.3 mW.cm-2) under continuous magnetic stirring for 10-12 hrs [10]. after irradiation, The obtained powder was separated and then the product was finally dried for 24h at 70°C. 2.2. Gram-negative bacteria exhibit only a thin peptidoglycan layer (ca. 2-3 nm) between the cytoplasmic membrane and the outer membrane, We believe that when the silver-doped Fe2O3 nanoparticles were dispersed in the growth media, the silver atoms present in these particles interacted with the bacterial cells and adhered to the bacterial cell walls.