Mass spectrometry proteomic diagnosis: enacting the validation paradigm

This paper presents an approach to the evaluation and validation of mass spectrometry data for construction of an `early warning' diagnostic procedure. We describe implementation of a designed experiment and place emphasis on the consistent and correct use of validation based evaluation - which...

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Bibliographic Details
Published inarXiv.org
Main Authors Mertens, Bart J A, de Noo, M E, R A E M Tollenaar, Deelder, A M
Format Paper
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 10.10.2005
Subjects
Cost analysis
Diagnosis
Diagnostic systems
Mass spectrometry
Scientific imaging
Spectroscopy
Statistical analysis
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Summary:This paper presents an approach to the evaluation and validation of mass spectrometry data for construction of an `early warning' diagnostic procedure. We describe implementation of a designed experiment and place emphasis on the consistent and correct use of validation based evaluation - which is a key requirement to achieve unbiased assessment of the ability of mass spectrometry data for diagnosis in this setting. Strict adherence to validation as a scientific principle will however typically imply that the analyst must make choices. Like all choices in statistical analysis, validation comes at a cost! We present a detailed and extensive discussion of the issues involved and propose that much greater emphasis and requirement for validation would enter clinical proteomic science.
ISSN:2331-8422