Binding Parameters of Alkaloids Berberine and Sanguinarine with DNA

We study the interaction of berberine and sanguinarine (plant alkaloids) with DNA in aqueous solutions, by using optical spectroscopy methods (absorption and fluorescence). The dependencies of alkaloid spectral characteristics on the concentration ratio N/c between the DNA base pairs and alkaloid mo...

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Bibliographic Details
Published inarXiv.org
Main Authors Gumenyuk, V G, Bashmakova, N V, S Yu Kutovyy, Yashchuk, V M, Zaika, L A
Format Paper
LanguageEnglish
Published Ithaca Cornell University Library, arXiv.org 11.01.2012
Subjects
Alkaloids
Aqueous solutions
Binding
Deoxyribonucleic acid
DNA
Fluorescence
Parameters
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Summary:We study the interaction of berberine and sanguinarine (plant alkaloids) with DNA in aqueous solutions, by using optical spectroscopy methods (absorption and fluorescence). The dependencies of alkaloid spectral characteristics on the concentration ratio N/c between the DNA base pairs and alkaloid molecules in the solutions are considered, and the manifestations of the alkaloid-DNA binding are revealed. The character of binding is found to depend on N/c. The parameters of the binding of berberine and sanguinarine with DNA are determined, by using the modified Scatchard and McGhee-von Hippel equations
ISSN:2331-8422