Optimized real-time qPCR assays for detecting and quantifying the Fusarium and Microdochium species responsible for wheat head blight, as defined by MIQE guidelines

Reliable real-time PCR assays for detecting and quantifying fungal species of the genera Fusarium and Microdochium are required, to improve our understanding of Fusarium head blight disease. We developed specific qPCR assays for nine species from these two genera. All the criteria required for assay...

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Bibliographic Details
Published inbioRxiv
Main Authors Elbelt, Sonia, Siou, Dorothee, Gelisse, Sandrine, Cruaud, Corinne, Lannou, Christian, Lebrun, Marc-Henri, Laval, Valerie
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 27.02.2018
Subjects
Deoxyribonucleic acid
DNA
Fusarium head blight
Polymerase chain reaction
Species
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Summary:Reliable real-time PCR assays for detecting and quantifying fungal species of the genera Fusarium and Microdochium are required, to improve our understanding of Fusarium head blight disease. We developed specific qPCR assays for nine species from these two genera. All the criteria required for assay reproducibility are presented. These assays are species-specific and have a quantification limit of 5 pg of fungal DNA/PCR and a detection limit of 0.5 pg of fungal DNA/PCR. Furthermore, the quantification performances of the tests were not affected by the presence of DNA from closely related species. The assays were tested on field samples and have already been used to analyze greenhouse experiments.
DOI:10.1101/272534