The RacGAP Chimaerin is essential for cerebellar granule cell migration

During mammalian cerebellar development, postnatal granule cell progenitors proliferate in the outer part of the External Granule Layer (EGL). Postmitotic granule progenitors migrate tangentially in the inner EGL before switching to migrate radially inward, past the Purkinje cell layer, to achieve t...

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Bibliographic Details
Published inbioRxiv
Main Authors Estep, Jason A, Wong, Wenny, Wong, Yiu-Cheung E, Loui, Brian M, Riccomagno, Martin M
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 19.12.2017
Subjects
Cell adhesion & migration
Cell migration
Cerebellum
Granule cells
Mossy fibers
Neural stem cells
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Summary:During mammalian cerebellar development, postnatal granule cell progenitors proliferate in the outer part of the External Granule Layer (EGL). Postmitotic granule progenitors migrate tangentially in the inner EGL before switching to migrate radially inward, past the Purkinje cell layer, to achieve their final position in the mature Granule Cell Layer (GCL). Here, we show that the RacGAP -chimaerin is expressed by a small population of late-born, premigratory granule cells. -chimaerin deficiency causes a subset of granule cells to become arrested in the EGL, where they differentiate and form ectopic neuronal clusters. These clusters of granule cells are able to recruit aberrantly projecting mossy fibers. Collectively, these data suggest a role for -chimaerin as an intracellular mediator of Cerebellar Granule Cell radial migration.
DOI:10.1101/164897