Identification of powdery mildew resistance QTL in Fragaria x ananassa

The obligate biotrophic fungus Podosphaera aphanis is the causative agent of powdery mildew on cultivated strawberry (Fragaria x ananassa). Genotypes from two bi-parental mapping populations "Emily" x "Fenella" and "Redgauntlet" x "Hapil" were phenotyped for p...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Cockerton, Helen M, Vickerstaff, Robert J, Wilson, Fiona, Karlstrom, Amanda, Sobczyk, Maria, Passey, Andy J, He, Joe Q, Mcleary, Kirsty J, Sargent, Daniel J, Pakozdi, Katalin, Harrison, Nicola, Martinez, Maria Lumbreras, Antanaviciute, Laima, Simpson, David W, Harrison, Richard J
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 18.01.2018
Subjects
Association analysis
Cultivars
Fragaria ananassa
Fungi
Gene mapping
Genotypes
Germplasm
Pedigree
Phenotyping
Plant breeding
Podosphaera aphanis
Powdery mildew
Quantitative trait loci
Online AccessGet full text

Cover

More Information
Summary:The obligate biotrophic fungus Podosphaera aphanis is the causative agent of powdery mildew on cultivated strawberry (Fragaria x ananassa). Genotypes from two bi-parental mapping populations "Emily" x "Fenella" and "Redgauntlet" x "Hapil" were phenotyped for powdery mildew disease severity in a series of field trials. Here we report multiple QTL associated with resistance to powdery mildew, identified in ten phenotyping events conducted across different years and locations. Seven QTL show a level of stable resistance across multiple phenotyping events however many other QTL were represented in a single phenotyping event and thus must be considered transient. One of the identified QTL was closely linked to an associated resistance gene across the wider germplasm. Furthermore, a preliminary association analysis identified a novel conserved locus for further investigation. Our data suggests that resistance is highly complex and that multiple additive sources of quantitative resistance to powdery mildew exist across strawberry germplasm. Implementation of the reported markers in marker-assisted breeding or genomic selection would lead to improved powdery mildew resistant strawberry cultivars, particularly where the studied parents, progeny and close pedigree material are included in breeding germplasm.
DOI:10.1101/196485