Enterococcus faecalis Modulates Immune Activation And Slows Healing During Wound Infection

Enterococcus faecalis is one of most frequently isolated bacterial species in wounds yet little is known about its pathogenic mechanisms in this setting. Here, we used a mouse wound excisional model to characterize the infection dynamics of E. faecalis and show that infected wounds result in two dif...

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Published inbioRxiv
Main Authors Kelvin Kian Long Chong, Wei Hong Tay, Janela, Baptiste, Mei Hui Adeline Yong, Tze Horng Liew, Madden, Leigh, Keogh, Damien, Barkham, Timothy, Ginhoux, Florent, Becker, David Laurence, Kline, Kimberly A
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 07.04.2017
Subjects
Bacterial proteins
Colonization
Cytokines
Fitness
Immune clearance
Immune response
Immunomodulation
Infections
Inflammation
Inoculum
Wound healing
Wound infection
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Summary:Enterococcus faecalis is one of most frequently isolated bacterial species in wounds yet little is known about its pathogenic mechanisms in this setting. Here, we used a mouse wound excisional model to characterize the infection dynamics of E. faecalis and show that infected wounds result in two different states depending on the initial inoculum. Low dose inocula were associated with short term, low titer colonization whereas high dose inocula were associated with acute bacterial replication and long term persistence. High dose infection and persistence were also associated with immune cell infiltration, despite suppression of some inflammatory cytokines and delayed wound healing. During high dose infection, the multiple peptide resistance factor (MprF) which is involved in resisting immune clearance, contributes to E. faecalis fitness. These results comprehensively describe a mouse model for investigating E. faecalis wound infection determinants, and suggest that both immune modulation and resistance contribute to persistent, non-healing wounds.
DOI:10.1101/125252