Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches

Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis,...

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Published inbioRxiv
Main Authors Babenko, Vladislav V, Podgorny, Oleg V, Manuvera, Valentin A, Kasianov, Artem S, Manolov, Alexander I, Grafskaia, Ekaterina N, Shirokov, Dmitriy A, Kurdyumov, Alexey S, Vinogradov, Dmitriy V, Nikitina, Anastasia S, Kovalchuk, Sergey I, Anikanov, Nickolay A, Butenko, Ivan O, Vakhitova, Maria T, Pobeguts, Olga V, Matushkina, Daria S, Rakitina, Daria V, Kostryukova, Elena S, Zgoda, Victor G, Trukhan, Vladimir M, Baskova, Isolda P, Gelfand, Mikhail S, Govorun, Vadim M, Schioth, Helgi B, Lazarev, Vassili N
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 10.09.2019
Subjects
Anticoagulants
Gene expression
Genomes
Hirudo medicinalis
Proteomics
Ribonucleic acid
RNA
Secretion
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Summary:Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific genes, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants were not differentially expressed in the salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. Thus, our study provides one of the most comprehensive knowledge of the genetic fundamentals of the blood-sucking lifestyle in leeches.
DOI:10.1101/357681