Germination-induced biluminescence, a route to determine the inhibitory effect of a combination preservation treatment on bacterial spores

In this work, we have used spores of Bacillus subtilis that specifically induce bioluminescence upon initiation of germination as a rapid, real-time monitor of the effects of preservative treatments on germination. Using this tool, we have demonstrated that the combination of mild acidity (pH 5.5 to...

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Bibliographic Details
Published inApplied and environmental microbiology Vol. 66; no. 9; p. 3735
Main Authors Ciarciaglini, Gianni, Hill, Philip J, Davies, Ken, McClure, Peter J
Format Journal Article
LanguageEnglish
Published Washington American Society for Microbiology 01.09.2000
Subjects
Bacteria
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Microbiology
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Summary:In this work, we have used spores of Bacillus subtilis that specifically induce bioluminescence upon initiation of germination as a rapid, real-time monitor of the effects of preservative treatments on germination. Using this tool, we have demonstrated that the combination of mild acidity (pH 5.5 to 5.0), lactic acid )0.5%), and a pasteurization step (90degreeC for 5 min) results in enhanced inhibition of spore germination compared with the effects of the individual treatments alone.
ISSN:0099-2240
1098-5336