Pollen Morphology and Characteristics of Rhododendron longipedicellatum(Ericaceae), an Endangered Species Endemic to Southeastern Yunnan, China / 滇东南濒危植物长梗杜鹃的花粉形态及其特性研究

• Published in: Forest research (Beijing) Vol. 31; no. 3; p. 51
• Main Authors: 李太强, 刘雄芳, 李正红, 万友名, 刘秀贤, 张序, 安静, 马宏
• Format: Journal Article
• Language: Chinese
• Published: Beijing Chinese Academy of Forestry 01.01.2018
• Subjects: Morphology; Sucrose
• ISSN: 1001-1498

[Objective] To provide some references for the studies on genetic breeding, pollen germplasm preservation and pollination biology of Rhododendron longipedicellatum by investigating the pollen grains morphology and its characteristics.[Method] The pollen grains of R. longipedicellatum at different development stages including alabastrum intumescence stage, petal loose stage and petal full bloom stage were observed by light microscope (LM) and scanning electron microscope (SEM). The pollen viability in the process of flowering was detected by TTC staining method. The effects of 10 g·L-1 agar and different concentrations of sucrose, H3BO3 and CaCl2 on R. longipedicellatum pollen grains germination were studied by L25(53) orthogonal test. Simultaneously, the authors also studied the changes of pollen germination rate under different storage temperature.[Result] The pollen grains mature gradually from the alabastrum intumescence to the petal full bloom stage, and are arranged in tetrahedral tetrad. The tetrad diam