Diagnostics and Detection of Different Groups Phytoplasmas in China Using an Oligonucleotide Microarray on the Platform of ArrayTube / 寡核苷酸管芯片技术检测和鉴别我国不同组植原体

• Published in: Forest research (Beijing) Vol. 30; no. 1; p. 99
• Main Authors: 王圣洁, 林彩丽, 严东辉, 于少帅, 李永, 汪来发, 朴春根, 郭民伟, 淮稳霞, 田国忠
• Format: Journal Article
• Language: Chinese
• Published: Beijing Chinese Academy of Forestry 01.01.2017
• Subjects: Candy industry; Cocoa; Plant diseases; China
• ISSN: 1001-1498
• DOI: 10.13275/j.cnki.lykxyj.2017.01.014

[Objective] d the optimal specific probe and develop the detection technique using oligonucleotide microarray on the platform of ArrayTube to detect and identify the phytoplasmas associated with plant disease in China.[Method] PCR amplification and microarray hybridization were used to detect 15 symptomatic plants probably infected with phytoplasma and asymptomatic plants as healthy controls collected from different regions in China.[Result] Phytoplasma 16S rDNA were detected in 13 of 15 symptomatic plants but not in all the healthy controls. Thirteen phytoplasmas were classified into 16Sr I, 16Sr II, 16Sr V and 16Sr XIX groups. Among 17 tested probes, the universal probe designated Pp-502 could be used to detect all phytoplasmas associated with plant disease. The specific probe designated Pp I-465 for 16Sr I group could be used to detect four phytoplasma strains of 16Sr I group associated with paulownia witches' broom, chinaberry witches' broom, mulberry dwarf and lettuce yellows. The probe Pp II-629 for 16S