Analysis of Clonal Growth and Differences in Gene Expression of Betula platyphylla×B.pendula under GA4+7 Treatment / 赤霉素GA4+7处理下白桦无性系生长及差异基因表达分析

• Published in: Forest research (Beijing) Vol. 30; no. 1; p. 181
• Main Authors: 刘宇, 徐焕文, 刘桂丰, 姜静
• Format: Journal Article
• Language: Chinese
• Published: Beijing Chinese Academy of Forestry 01.01.2017
• Subjects: Gene expression; Phosphorylation
• ISSN: 1001-1498
• DOI: 10.13275/j.cnki.lykxyj.2017.01.025

[Objective] lore the regulation mechanism of gibberellin GA4+7 on the growth of birch.[Method] The experiment was carried out by using two treatments from a tissue-cultured plantlet. The leaf surface was treated by foliar spraying GA4+7 solution with concentrations of 0.2 g·L-1 (T1) and 0.4 g·L-1 (T2) for two consecutive years, respectively. Then the differential gene and transcriptome sequencing of the corresponding top growth tissue were analyzed.[Result] It was found that the difference of height and ground diameter of the plantlet under different treatments reached significant or extremely significant level after two years. It is proved that the GA4+7 has a significant promoting effect on height growth. The height of birch seedlings under T2 treatment increased by 19.65% compared with the CK. The results of RNA-seq analysis of birch bud for treated by GA4+7 for continuous 2 years showed that the number of different gene was the most between the 0.4 g·L-1 of GA4+7 (T2) treatment and the CK, a total of 181