Immobilised-β-galactosidase Catalysed Conversion of Lactose on the Membrane Surface

Galacto-oligosaccharides (GOS) can be synthesised via ß-galactosidase (ß-Gal) catalysed conversion of lactose. The reaction process was carried out in the membrane reactor system to improve the conversion by partial separation of the products and galactose inhibitor. The reaction rate was determined...

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Bibliographic Details
Published inJournal of physical science Vol. 29; pp. 49 - 56
Main Authors Gonawan, Fadzil Noor, Kamaruddin, Azlina Harun, Abu Bakar, Mohamad Zailani, Karim, Khairiah Abd
Format Journal Article
LanguageEnglish
Published Pinang Universiti Sains Malaysia Press 01.01.2018
Subjects
Adsorption
Chemical synthesis
Conversion
Enzymes
Galactose
Galactosidase
Glucose
Inhibitors
Lactose
Mass transfer
Membrane reactors
Oligosaccharides
Separation
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Summary:Galacto-oligosaccharides (GOS) can be synthesised via ß-galactosidase (ß-Gal) catalysed conversion of lactose. The reaction process was carried out in the membrane reactor system to improve the conversion by partial separation of the products and galactose inhibitor. The reaction rate was determined by the concentration of the solutes on the biocatalytic membrane and it was varied by manipulating the trans-membrane pressure (TMP). The yield of GOS was highest at a TMP = 0.5 bar. It was found that the formation of gel layer at higher TMP (> 0.5 bar) reduced the mass transfer of solutes through the biocatalytic membrane. Therefore, the reaction rates were decreased although the separation of galactose inhibitor was improved. Hence, it is concluded that the mass transfer of solute is the determining factor to obtain a high yield of GOS.
ISSN:1675-3402
2180-4230
DOI:10.21315/jps2018.29.sL7