WS Immunodetection of the Cry toxin in leaves of transgenic maize hybrids

• Published in: Journal of animal science Vol. 94; p. 305
• Main Authors: Neto, G Balieiro, Freitas, A W P, Branco, R Botelho Ferraz, Duarte, K Maria Roncato, Pela, F Porto, Baruffi, M D
• Format: Journal Article
• Language: English
• Published: Champaign Oxford University Press 01.10.2016
• Subjects: Antibodies; Calibration; Corn; Crops; Cultivation; Enzyme-linked immunosorbent assay; Experimental design; Hybrids; Infestation; Information processing; Pests; Planting; Studies; Toxins; Transgenic plants
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/jam2016-0639

This assay aimed to evaluate the Cry toxins concentration in the last completely expanded leaf at 7, 14, 28, 35, 70, 84, and 96 d after planting of five corn hybrids. The evaluated hybrids were Syngenta Impact TL TG, Monsanto DKB 390 VT PRO II, Monsanto AG 8088 PRO II, Biomatrix 2B655 Herculex from Dow, and Syngenta 7205 Viptera. To analyze the toxin concentrations, antibodies were produced and a PTA-ELISA was developed. The positive controls used to produce the calibration curves were Sigma-Aldrich BF412b (Cry1Ab), Sigma-Aldrich BF418b (Cry1F), and Sigma-Aldrich BF423b (Vip3Aa20). The experimental design was randomized blocks in a factorial arrangement 5 (hybrid) × 7 (samples) with five repetitions. There were a significant interaction between hybrid and sampling period. The mean concentrations of the Cry toxins in the hybrids Herculex, AG 8088 PRO II, DKB 390 VT PRO II, Viptera, and Impacto were 7.58, 13.05, 16.81, 22.67, and 34.05 μg/g of fresh tissue, respectively. The mean concentration of Cry toxins were 16.77, 15.16, 18.46, 22.87, 22.85, 20.62, and 15.98 μg/g of fresh tissue at 7, 14, 28, 35, 70, 84, and 96 d after planting. Higher concentration of Cry toxin occurred between 35 and 70 d after planting and the hybrids with the higher concentrations were Viptera and Impacto with concentrations of 34.6 and 39.6 μg/g of fresh tissue, respectively. This information can integrate selection criteria of hybrids for cultivation in different regions according to the level of infestation of pests and resistance thereof to different Cry toxin concentrations.