Production of bile acid salts binding hydrolysate from Trachinotus ovatus and its molecular weight distribution

Taking ovate pompano(Trachinotus ovatus) meat as raw material and taking enzymatic hydrolysate and bile salts in vitro binding rate as index,we screened the optimal protease from five kinds of food protease.Then we used orthogonal design to optimize hydrolysis conditions in order to improve the bind...

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Bibliographic Details
Published inNanfang shuichan kexue no. 2
Main Authors Zhu, Xiaolian, Chen, Hua, Cai, Bingna, Wan, Peng, Sun, Huili, Chen, Deke, Pan, Jianyu
Format Journal Article
LanguageChinese
Published Guangzhou Chinese Academy of Fishery Sciences 01.01.2017
Subjects
Acids
Additives
Bile
Distribution
Enzymolysis
Hydrates
Meat
Molecular weight
Peptides
Proteins
Raw materials
Salts
Sodium
Trachinotus ovatus
Weight
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Summary:Taking ovate pompano(Trachinotus ovatus) meat as raw material and taking enzymatic hydrolysate and bile salts in vitro binding rate as index,we screened the optimal protease from five kinds of food protease.Then we used orthogonal design to optimize hydrolysis conditions in order to improve the binding rate of bile acid salts and used GPC method to analyze the molecular weight distribution of peptides.The results show that the tryptic hydrolysates had higher binding rate than that of the other protease hydrolysates,and the in vitro binding rate of sodium cholate,sodium glycocholate and sodium taurocholate by enzymatic protein(100 mg·m L~(-1))was 42.1%,33.5% and 30.1%,respectively,which was 78.3%,74.4% and 76.8% equivalent to the positive control cholestyramine(20 mg·m L~(-1)).The maximum percentage of sodium glycocholate hydrate binding rate was obtained under the conditions:hydrolytic time of 2 h,enzyme additives of 2 000 U·g~(-1),meal-to-water ratio of 1∶4(g·m L~(-1),at p H 8,37 ℃).Under the optimal conditi
ISSN:2095-0780