High and Ultra Low Concentrations of Sodium Chloride Initiate their Action on Binding Sites of a Protein

There are clinical and experimental evidences which show that ultra low concentrations (ULC) of drugs used in homeopathy produce biological effects. ULCs differ from each other with respect to free water molecules or OH groups and H-bond strength of OH groups. The objective is to see whether ULCs of...

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Bibliographic Details
Published inEnvironment and ecology Vol. 36; no. 1A; p. 209
Main Authors Sarkar, Tandra, Konar, Atheni, Sukul, Nirmal Chandra, Sukul, Anirban
Format Journal Article
LanguageEnglish
Published Kalyani MKK Publications 01.01.2018
Subjects
Binding sites
Biological effects
Bond strength
Bonding strength
Bovine serum albumin
Calorimetry
Control equipment
Distilled water
Drag
Endothermic reactions
Enthalpy
Entropy
Exothermic reactions
Free energy
Homeopathic medicine
Low concentrations
Serum albumin
Sodium
Sodium chloride
Water chemistry
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Summary:There are clinical and experimental evidences which show that ultra low concentrations (ULC) of drugs used in homeopathy produce biological effects. ULCs differ from each other with respect to free water molecules or OH groups and H-bond strength of OH groups. The objective is to see whether ULCs of a drag, Natrum mur and its high concentration (0) initiate their action on the binding sites of a protein, bovine serum albumin (BSA). Nat-m ? (500 m M NaCl solution in water) and its 2 potencies 30 cH (100 µM) and 200 cH (100 µM) were prepared in the laboratory. The control was distilled water (55 mM). The drag and control solutions were injected separately 20 times at 6 µl/injection every 2 min into a sample cell containing 7 µM BSA at 25°C in the isothermal calorimetry (ITC) instrument. While Nat-m ? produced endothermic reaction, the potencies and the control produced exothermic reaction. ITC parameters binding constant (K), change in enthalpy (?H), entropy (?S) and free energy (?G) varied markedly from each other among the control and drag solutions. Control, Nat-m ?, 30 cH and 200 cH showed single site, sequential interaction in 3 sites, same in 3 sites and sequential interaction in 4 sites of BSA, respectively. It is concluded that the drug and the control initiate their action on binding sites of BSA. Nat-m ? and its potencies produced endothermic and exothermic reaction, respectively on BSA. Repetition of a dose produced increasing saturation.
ISSN:0970-0420