The polymorphism of RseI type at STAT 5A locus in cattle

The association between the polymorphisms at different gene loci and milk traits is studied in many cattle breeds. Our study aimed to perform genotypization at STAT 5A locus in Romanian Black Spotted cattle (BNR = Bălţată cu Negru Românească), individuals with big milk production (BNR-M) and small m...

Full description

Saved in:
Bibliographic Details
Published inAnimal biology & animal husbandry Vol. 5; no. 2; pp. 236 - 240
Main Authors Carşai, Teodora C, Vlaic, Augustin
Format Journal Article
LanguageEnglish
Published Cluj-Napoca Bioflux SRL 01.12.2013
Subjects
Alleles
Animal lactation
Animal sciences
Bovidae
Cattle
Deoxyribonucleic acid
DNA
Exons
Gene frequency
Gene loci
Gene mapping
Genetic analysis
Genetics
Genotypes
Growth hormones
Milk
Milk production
Mutation
Polymerase chain reaction
Proteins
Restriction fragment length polymorphism
Zoology
United States > US
Online AccessGet full text

Cover

More Information
Summary:The association between the polymorphisms at different gene loci and milk traits is studied in many cattle breeds. Our study aimed to perform genotypization at STAT 5A locus in Romanian Black Spotted cattle (BNR = Bălţată cu Negru Românească), individuals with big milk production (BNR-M) and small milk production (BNR-S), in order to identify the gene and allele frequency. In order to perform the genotypization at STAT 5A locus in cattle, specific primer sets were used (some described by literature, other new synthesized). They allowed the amplification of exons from genes where mutations were identified. The genetic analysis was performed using PCR-RFLP. For each amplification products possible to be obtained from polymorphic regions, based on mutations, there were performed theoretic restriction maps with NEBcutter V2.0. The results of our study show that CT genotype and allele C were identified only in BNR-M genotype.
ISSN:2066-7612
2067-6344