Cross-presenting human [gamma][delta] T cells induce robust CD8+ [alpha][beta] T cell responses

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 7; p. 2307
• Main Authors: Brandes, Marlène, Willimann, Katharina, Bioley, Gilles, Lévy, Nicole, Eberl, Matthias, Luo, Ming, Tampé, Robert, Lévy, Frédéric, Romero, Pedro, Moser, Bernhard
• Format: Journal Article
• Language: English
• Published: Washington National Academy of Sciences 17.02.2009
• Subjects: Cytokines; Immunotherapy; Molecules; Studies; T cell receptors; Tumors
• ISSN: 0027-8424; 1091-6490

... T cells are implicated in host defense against microbes and tumors but their mode of function remains largely unresolved. Here, we have investigated the ability of activated human ... T cells (termed ... T-APCs) to cross-present microbial and tumor antigens to CD+ αβ T cells. Although this process is thought to be mediated best by DCs, adoptive transfer of ex vivo antigen-loaded, human DCs during immunotherapy of cancer patients has shown limited success. We report that ... T-APCs take up and process soluble proteins and induce proliferation, target cell killing and cytokine production responses in antigen-experienced and naive CD8+ αβ T cells. Induction of APC functions in ... T cells was accompanied by the up-regulation of costimulatory and MHC class I molecules. In contrast, the functional predominance of the immunoproteasome was a characteristic of gd T cells irrespective of their state of activation. ... T-APCs were more efficient in antigen cross-presentation than monocytederived DCs, which is in contrast to the strong induction of CD4+ ab T cell responses by both types of APCs. Our study reveals unexpected properties of human ... TAPCs in the induction of CD8+ ab T effector cells, and justifies their further exploration in immunotherapy research. (ProQuest: ... denotes formulae/symbols omitted.)