Effects of miR-451 on the biological behavior of colorectal carcinoma cell line SW620

Objective To explore the effects of human miR-451 on the proliferation,apoptosis and invasive ability of colon cancer cell line SW620.Methods Four groups were designed in the present experiment,i.e.,cells were transfected with 100nmol/L of miR-451 mimics in miR-451 mimics group,with 100nmol/L of neg...

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Bibliographic Details
Published inJie fang jun yi xue za zhi Vol. 36; no. 5; p. 478
Main Authors Chun-rong, WU, LUO, Zhi-bin, CHEN, Wei
Format Journal Article
LanguageChinese
Published Beijing People's Military Medical Press 01.01.2011
Subjects
Colorectal cancer
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Summary:Objective To explore the effects of human miR-451 on the proliferation,apoptosis and invasive ability of colon cancer cell line SW620.Methods Four groups were designed in the present experiment,i.e.,cells were transfected with 100nmol/L of miR-451 mimics in miR-451 mimics group,with 100nmol/L of negative control in negative control(NC) group,with Lipo 2000 alone in blank control group,and without transfection of either Lipo2000 or miRNA in control group.The expression of miR-451 was measured by real-time RT-PCR 24h after transfection,and proliferation of cells was examined by using the Cell Counting Kit-8 at respective time of 24,48,72 hours after transfction.The apoptotic rate was assessed by flow cytometry(FCM) and invasive ability was determined by Transwell assay.The effect of miR-451 mimics on the expression of macrophage migration inhibitory factor(MIF) protein was measured by immunofluorescent and Western blot assay.Results Twenty-four hours after transfection,an increase in expression of miR-451 was n
ISSN:0577-7402