Damage of rat liver tissue caused by repeated and sustained +Gz exposure and the mechanism thereof

• Published in: Jie fang jun yi xue za zhi Vol. 39; no. 3; p. 240
• Main Authors: Wen-bing, LI, Ya-lin, KONG, Xiao-jun, HE, ZHAO, Gang, Huai-cheng ZHU, HU, Shen, LIU, Lei, Hong-yi, ZHANG
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2014
• Subjects: Liver; Protein expression; Proteins; Rodents
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2014.03.15

Objective 　To explore the mechanisms of positive acceleration (+Gz) on the damage of rat liver tissue and the effect of +Gz on the expression of JNK/c-Jun in liver cells. Methods 　Twenty four male Wistar rats were randomly divided into 4 groups (n=6): control, +2Gz, +6Gz and +10Gz group. With prone position, the rats in control group were fixed to the turning arm of centrifuge with head towards the axis for 5 minutes. The fixation method in +2Gz, +6Gz and +10Gz group was the same as in the control group. The increase rate of acceleration was 1G/s with a peak-time of 3 minutes, and each +Gz exposure repeated 5 times with an interval of 30 minutes. HE staining was used to observe the morphological changes of liver tissue, fluorescence real-time quantitative PCR to detect the expression of hepatic c-Jun mRNA, and Western blotting to detect the hepatic protein expression of p-c-Jun, c-Jun, p-JNK and JNK. Plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined. Results 　The level