Repeated Aerosol Delivery of Carboxyl-terminal Modulator Protein Suppresses Tumor in the Lungs of K-ras^sup LA1^ Mice

• Published in: American journal of respiratory and critical care medicine Vol. 179; no. 12; p. 1131
• Main Authors: Hwang, Soon-Kyung, Lim, Hwang-Tae, Minai-Tehrani, Arash, Lee, Eun-Sun, Park, Jongmin, Park, Seung Bum, Beck, George R, Cho, Myung-Haing
• Format: Journal Article
• Language: English
• Published: New York American Thoracic Society 15.06.2009
• ISSN: 1073-449X; 1535-4970

Difficulties in achieving long-term survival of patients with lung cancer treated with conventional therapies suggest that novel approaches are required. Recent advances in aerosol-mediated gene delivery have provided the possibility of an alternative for the safe and effective treatment of lung cancer. To investigate the repeated effect of carboxyl-terminal modulator protein (CTMP) on multistage lung tumorigenesis. In this study, we addressed this question by studying the effects of lentivirus-based CTMP in the lungs of 9- and 13-week-old K-ras(LA1) mice, a model of lung cancer. An aerosol of lentivirus-based CTMP was delivered into 9- and 13-week-old K-ras(LA1) mice, a model of lung cancer, through a nose-only inhalation system twice a week for 4 weeks. The effects of CTMP on lung cancer progression and Akt-related signals were evaluated. Long-term repeated delivery of CTMP effectively reduced tumor progression in the lungs at different stages of development. Lentiviral-CTMP inhibited protein synthesis and cell cycle and altered Akt signaling pathway in the lungs of 9-week-old K-ras(LA1) mice, and increased apoptosis was observed in the lungs of 13-week-old K-ras(LA1) mice. Long-term repeated viral delivery of CTMP may provide a useful tool for designing lung tumor treatment.