Antibacterial activity in vitro of essential oils from different species of the genus Citrus

• Published in: Revista colombiana de ciencias químico-farmacéuticas Vol. 46; no. 2
• Main Authors: Miladys Esther Torrenegra Alarcón, Nerlis, Paola Pájaro, Glicerio León Méndez
• Format: Journal Article
• Language: Spanish
• Published: Bogota Universidad Nacional de Colombia 01.01.2017
• Subjects: Agar; Aromatherapy; Bacteria; Broths; E coli; Essential oils; Klebsiella; Oils & fats; Optical density; Pseudomonas aeruginosa
• ISSN: 0034-7418; 1909-6356
• DOI: 10.15446/rcciquifa.v46n2.67934

The in vitro antibacterial activity of essential oils of different species of the Citrus genus was evaluated against ATCC strains of Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The bacteria were replicated in specific agar media and broths. The maximum optical density moment (OD620) was determined to be used as the incubation time; sensitivity tests were then performed with exposure of the strains at concentrations to 1000 g/mL broth extract. 1% dimethylsulfoxide (DMSO) was used to solubilize. Subsequently, the minimum inhibitory concentration was determined using microdilution methodologies in broth and the minimum bactericidal concentration. We found strong activity of the essential oils of the genus Citrus, with values of CMI ≥ 600 mg/mL against S. aureus, S. epidermidis, K. pneumoniae, P. aeruginosa and E. coli. Based on the results obtained, it is concluded that the different species of Citrus genus are considered as promising for the control of the bacterial component.