DNA barcoding of ornamental fishes of Jammu

Background: Ornamental fish, also known as aquarium fish, are called living jewels. They have different colour combinations on their bodies, attractive body shapes, and numerous fin structures, which make them objects of considerable aesthetic value. Identification of ornamental fish in Jammu and Ka...

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Bibliographic Details
Published inGenome Vol. 60; no. 11; p. 907
Main Authors Arif, Mohd M, Gandotra, Roopma GP
Format Journal Article
LanguageEnglish
Published Ottawa Canadian Science Publishing NRC Research Press 01.11.2017
Subjects
Alcohols
Aquariums
Conservation
Cytochrome-c oxidase
Deoxyribonucleic acid
DNA
DNA barcoding
DNA sequencing
Fish
Fish conservation
High altitude
Identification methods
Jewelry
Lakes
Mitochondria
Nets
Ornamental fish
Perennial streams
Polymerase chain reaction
Primers
Rivers
Species
Streams
Taxonomy
Warm water
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Summary:Background: Ornamental fish, also known as aquarium fish, are called living jewels. They have different colour combinations on their bodies, attractive body shapes, and numerous fin structures, which make them objects of considerable aesthetic value. Identification of ornamental fish in Jammu and Kashmir State can be challenging, especially due to the different distinct water conditions, which include both cold and warm water streams, perennial rivers, lakes, and reservoirs. There are also 250 high altitude lakes spread over an area of 40 000 ha. DNA barcoding, which uses the mitochondrial cytochrome c oxidase 1 (COI) gene as a target gene, is an efficient and accurate method for fish identification for assessment and conservation of dwindling diversity. Results: A survey of various water bodies in and around Jammu was carried out for collection of different fish species available. Fish were caught from slow-moving streams and ponds with the help of drag nets and cast nets, and preserved in 10% formalin and 100% alcohol. Identification of collected ornamental fish was done using traditional taxonomic methods. DNA extraction, PCR amplification with species-specific PCR primers, and DNA sequencing will be carried out, which will offer great potential for the identification of species. Significance: A first attempt has been made to create a database of DNA barcodes that will be helpful in identification of fishes and also to authenticate the diversity of ornamental fish present in Jammu areas. This study will prove a milestone in advanced research by using modern techniques and easy to handle methods for fish identification in different geographical limits. It will also address some taxonomic issues that need further investigation.
ISSN:0831-2796
1480-3321